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Criblage à haut débit d'Enzymes Glucides dégradant Utilisation Novel Insoluble chromogénique Substrat Assay Kits
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Bioquímica
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JoVE Journal Bioquímica
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
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Criblage à haut débit d'Enzymes Glucides dégradant Utilisation Novel Insoluble chromogénique Substrat Assay Kits

12,536 Views

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06:51 min

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September 20, 2016

DOI:

10.3791/54286-v

DOI:
10.3791/54286-v

•

06:51 min
September 20, 2016

•

5 Views
, ,
1Department for Plant and Environmental Sciences,University of Copenhagen, 2School of Agriculture, Food and Rural Development,Newcastle University

Summary

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A high-throughput assay for enzyme screening is described. This multiplexed ready-to-use assay kit comprises of pre-chosen Chromogenic Polymer Hydrogel (CPH) substrates and complex Insoluble Chromogenic Biomass (ICB) substrates. Target enzymes are polysaccharide degrading endo-enzymes and proteases.

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Schückel, J., Kračun, S. K., Willats, W. G. T. High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits. J. Vis. Exp. (115), e54286, doi:10.3791/54286 (2016).

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