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Los ensayos basados en la microscopía para la selección de alto rendimiento de los factores del huésped que participan en<em> Brucella</em> Infección de células Hela
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Los ensayos basados en la microscopía para la selección de alto rendimiento de los factores del huésped que participan en<em> Brucella</em> Infección de células Hela
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August 05, 2016
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Summary
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Two assays for microscopy-based high-throughput screening of host factors involved in Brucella infection are described. The entry assay detects host factors required for Brucella entry and the endpoint assay those required for intracellular replication. While applicable for alternative approaches, siRNA screening in HeLa cells is used to illustrate the protocols.
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Casanova, A., Low, S. H., Emmenlauer, M., Conde-Alvarez, R., Salcedo, S. P., Gorvel, J., Dehio, C. Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells. J. Vis. Exp. (114), e54263, doi:10.3791/54263 (2016).
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