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从人类肿瘤异种移植的小鼠细胞的消耗显着改善靶细胞下游分析
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July 29, 2016
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人类肿瘤异种移植是血管，并在体内生长阶段由鼠源细胞浸润。为了规避下游分析过程中造成这些污染的细胞偏差，我们开发了允许通过磁性细胞分选所有小鼠细胞的全面枯竭的方法。
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Agorku, D. J., Tomiuk, S., Klingner, K., Wild, S., Rüberg, S., Zatrieb, L., Bosio, A., Schueler, J., Hardt, O. Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells. J. Vis. Exp. (113), e54259, doi:10.3791/54259 (2016).
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