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Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
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July 29, 2016
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Human tumor xenografts are vascularized and infiltrated by cells of mouse origin during the growth phase in vivo. To circumvent the bias caused by these contaminating cells during downstream analysis, we developed a method allowing for comprehensive depletion of all mouse cells by magnetic cell sorting.
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Agorku, D. J., Tomiuk, S., Klingner, K., Wild, S., Rüberg, S., Zatrieb, L., Bosio, A., Schueler, J., Hardt, O. Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells. J. Vis. Exp. (113), e54259, doi:10.3791/54259 (2016).
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