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תרבות של Macrophage מושבה מגרה פקטור בדיל אדם המקרופאגים מונוציטים הנגזר
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

תרבות של Macrophage מושבה מגרה פקטור בדיל אדם המקרופאגים מונוציטים הנגזר

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06:46 min

June 30, 2016

DOI:

06:46 min
June 30, 2016

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Summary

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A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.

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