Journal
/
/
Human Primary trofoblasten Cell Culture modell för att studera de skyddande effekterna av Melatonin mot hypoxi / ny syresättning-inducerad Störningar
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
DOI:

12:02 min

July 30, 2016

, , , , , , , , ,

Capítulos

  • 00:05Título
  • 01:43Villous Cytotrophoblast Isolation
  • 05:51Trophoblast Purification
  • 08:25In Vitro Induction of Normoxia and Hypoxia/reoxygenation
  • 09:24Results: Anti-oxidant Effect of Melatonin in Syncytiotrophoblast Exposed to Hypoxia/reoxygenation
  • 10:47Conclusion

Summary

Tadução automática

This manuscript presents a unique in vitro model of immunopurified human villous cytotrophoblast cells cultured under hypoxia/reoxygenation. This model is suitable to study the protective effects of promising treatments, such as melatonin, on pregnancy complications associated with increased oxidative stress and altered placental function.

Vídeos Relacionados

Read Article