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Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
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JoVE Journal Biologia dello sviluppo
Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption

Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption

DOI:
10.3791/54228-v

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12:02 min

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July 30, 2016

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, , , , , , , , ,
1INRS-Institut Armand-Frappier

Capitoli

  • 00:05Titolo
  • 01:43Villous Cytotrophoblast Isolation
  • 05:51Trophoblast Purification
  • 08:25In Vitro Induction of Normoxia and Hypoxia/reoxygenation
  • 09:24Results: Anti-oxidant Effect of Melatonin in Syncytiotrophoblast Exposed to Hypoxia/reoxygenation
  • 10:47Conclusion

Summary

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This manuscript presents a unique in vitro model of immunopurified human villous cytotrophoblast cells cultured under hypoxia/reoxygenation. This model is suitable to study the protective effects of promising treatments, such as melatonin, on pregnancy complications associated with increased oxidative stress and altered placental function.

Tags

Keywords: Primary Trophoblast Cell CultureHypoxia/reoxygenationMelatoninPregnancy ComplicationsPlacentaOxidative StressVillous Cytotrophoblast IsolationDensity CentrifugationTrophoblast CultureFirst Trimester Placenta

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