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Menneskelig Primær trofoblasten Cell Culture Model at studere de beskyttende virkninger af melatonin Against Hypoksi / reoxygenering-induceret Forstyrrelse
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
DOI:

12:02 min

July 30, 2016

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Capítulos

  • 00:05Título
  • 01:43Villous Cytotrophoblast Isolation
  • 05:51Trophoblast Purification
  • 08:25In Vitro Induction of Normoxia and Hypoxia/reoxygenation
  • 09:24Results: Anti-oxidant Effect of Melatonin in Syncytiotrophoblast Exposed to Hypoxia/reoxygenation
  • 10:47Conclusion

Summary

Tadução automática

This manuscript presents a unique in vitro model of immunopurified human villous cytotrophoblast cells cultured under hypoxia/reoxygenation. This model is suitable to study the protective effects of promising treatments, such as melatonin, on pregnancy complications associated with increased oxidative stress and altered placental function.

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