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蛋白质生物缀合物的合成<em>通过</em>半胱氨酸马来酰亚胺化学

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Química

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JoVE Journal Química

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

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蛋白质生物缀合物的合成<em>通过</em>半胱氨酸马来酰亚胺化学

DOI:

10.3791/54157-v

•

09:14 min

•

July 20, 2016

•

Alexander F. Mason, Pall Thordarson

¹School of Chemistry, The Australian Centre for Nanomedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology,The University of New South Wales

Capítulos

00:05Título
00:43Purification of Cytochrome c
02:11Synthesis of Cytochrome c Bioconjugates
03:47Purification of Cytochrome c Bioconjugates
06:02Investigation of Bioconjugates by Gel Electrophoresis
07:08Results: Synthesis and Characterization of Cytochrome c Bioconjugates
08:02Conclusion

Summary

Tadução automática

该协议细节对于含有蛋白质马来酰亚胺，包括试剂纯化，反应条件，生物共轭纯化和生物共轭表征半胱氨酸的生物结合所需的重要步骤。

Tags

Keywords: Protein Bioconjugates Cysteine-maleimide Chemistry Site-specific Modification Antibody Drug Conjugates Nano-medicine Fluorescent Microscopy Systems Chemistry Cytochrome C FPLC Ruthenium Maleimide Complex TCEP

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