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为记录细胞内电压响应电法<em>果蝇</em>光感受器和中间对光刺激<em>在体内</em
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo
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为记录细胞内电压响应电法<em>果蝇</em>光感受器和中间对光刺激<em>在体内</em

DOI:
10.3791/54142-v

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11:42 min

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June 19, 2016

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, , ,
1National Key Laboratory of Cognitive Neuroscience and Learning,Beijing Normal University, 2Department of Biomedical Science,The University of Sheffield

Capítulos

  • 00:05Título
  • 00:52Fabricating Microelectrode
  • 02:39Drosophila Preparation
  • 05:34Recording from R1-R6 Photoreceptors or LMCs
  • 08:50Results: Applications
  • 10:27Conclusion

Summary

Tadução automática

Tadução automática

Sharp microelectrodes enable accurate electrophysiological characterization of photoreceptor and visual interneuron output in living Drosophila. Here we show how to use this method to record high-quality voltage responses of individual cells to controlled light stimulation. This method is ideal for studying neural information processing in insect compound eyes.

Tags

Keywords: Intracellular VoltageDrosophilaPhotoreceptorsInterneuronsLight StimuliElectrophysiologicalRecordingMicroelectrodeBorosilicateQuartz GlassPipette PullerReference ElectrodeRecording ElectrodeFly RingersPotassium ChloridePotassium AcetateChloride BatteryFly VialElectrophysiology

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