Journal
/
Genética
/
为患者组织甲基结合DNA序列捕获
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Methyl-binding DNA capture Sequencing for Patient Tissues
Please note that all translations are automatically generated. Click here for the English version.

为患者组织甲基结合DNA序列捕获

8,405 Views

•

08:40 min

•

October 31, 2016

DOI:

10.3791/54131-v

DOI:
10.3791/54131-v

•

08:40 min
October 31, 2016

•

14 Views
, , , , , , ,
1Department of Molecular Medicine,University of Texas Health Science Center at San Antonio, 2Greehey Children’s Cancer Research Institute,University of Texas Health Science Center at San Antonio

Summary

Automatically generated

在这里，我们提出了一个协议，调查使用甲基结合DNA测序捕获（MBDCap-SEQ或MBD-SEQ）技术以及随后的生物信息学分析管道的大规模临床病人的筛查研究全基因组DNA甲基化。

Explore More Videos

DNA MethylationEpigeneticsPatient CohortsBiomarkersMethyl-binding DNA CaptureSequencingGenome-wide AnalysisCost-effectiveSonicationFragment SizeElutionMagnetic Beads

Read Article

Cite this Article

Jadhav, R. R., Wang, Y. V., Hsu, Y., Liu, J., Garcia, D., Lai, Z., Huang, T. H. M., Jin, V. X. Methyl-binding DNA capture Sequencing for Patient Tissues. J. Vis. Exp. (116), e54131, doi:10.3791/54131 (2016).

Download .ris file

Copy

Share Video

.

Copy