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Stripe Assay aan de Aantrekkelijke of Repulsive activiteit van een eiwit ondergrond met Gescheiden hippocampus Neuronen Studie
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Stripe Assay aan de Aantrekkelijke of Repulsive activiteit van een eiwit ondergrond met Gescheiden hippocampus Neuronen Studie
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June 19, 2016
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Axon guidance molecules regulate neuronal migration and targeted growth-cone navigation. We present a powerful method, the stripe assay, to assess the ability of guidance molecules to attract or repulse neurons. In this protocol, we demonstrate the stripe assay by showing FLRT2's ability to repel cultured hippocampal neurons.
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Yamagishi, S., Kesavamoorthy, G., Bastmeyer, M., Sato, K. Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons. J. Vis. Exp. (112), e54096, doi:10.3791/54096 (2016).
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