Journal
/
Imunologia e Infecção
/
EAK 16-II / EAKIIH6自己組織化ヒドロゲルとFACS精製した胸腺上皮細胞の3-D集約を推進
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel
Please note that all translations are automatically generated. Click here for the English version.

EAK 16-II / EAKIIH6自己組織化ヒドロゲルとFACS精製した胸腺上皮細胞の3-D集約を推進

6,338 Views

•

08:02 min

•

June 27, 2016

DOI:

10.3791/54062-v

DOI:
10.3791/54062-v

•

08:02 min
June 27, 2016

•

12 Views
, , , , , , , ,
1Institute of Cellular Therapeutics,Allegheny Health Network, 2Division of Pharmaceutical Sciences, Mylan School of Pharmacy,Duquesne University, 3Department of Biological Sciences,Carnegie Mellon University

Summary

Automatically generated

This video demonstrates a protocol to enrich thymic epithelial cells (TECs) with density gradient for FACS isolation. It also shows the use of EAK16-II/EAKIIH6 peptides to promote the TEC aggregate formation. The microenvironments of EAK16-II/EAKIIH6 hydrogel provide the 3-D configuration necessary to maintain the survival and function of the TECs.

Explore More Videos

3-D AggregationThymic Epithelial CellsFACS PurificationEAK 16-II/EAKIIH6 Self-assembling HydrogelThymus BioengineeringCell HarvestingEnzymatic DigestionCell WashingCell Suspension

Read Article

Cite this Article

Tajima, A., Liu, W., Pradhan, I., Bertera, S., Lakomy, R. A., Rudert, W. A., Trucco, M., Meng, W. S., Fan, Y. Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel. J. Vis. Exp. (112), e54062, doi:10.3791/54062 (2016).

Download .ris file

Copy

Share Video

.

Copy