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Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel
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JoVE 杂志 免疫与感染
Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel

Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel

6,347 Views

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08:02 min

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June 27, 2016

DOI:

10.3791/54062-v

DOI:
10.3791/54062-v

•

08:02 min
June 27, 2016

•

12 Views
, , , , , , , ,
1Institute of Cellular Therapeutics,Allegheny Health Network, 2Division of Pharmaceutical Sciences, Mylan School of Pharmacy,Duquesne University, 3Department of Biological Sciences,Carnegie Mellon University

Summary

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This video demonstrates a protocol to enrich thymic epithelial cells (TECs) with density gradient for FACS isolation. It also shows the use of EAK16-II/EAKIIH6 peptides to promote the TEC aggregate formation. The microenvironments of EAK16-II/EAKIIH6 hydrogel provide the 3-D configuration necessary to maintain the survival and function of the TECs.

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Tajima, A., Liu, W., Pradhan, I., Bertera, S., Lakomy, R. A., Rudert, W. A., Trucco, M., Meng, W. S., Fan, Y. Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel. J. Vis. Exp. (112), e54062, doi:10.3791/54062 (2016).

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