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Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques
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Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques
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June 01, 2016
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This article describes a protocol for visualizing amyloid Aβ plaques in Alzheimer's disease mouse models using methoxy-X04, which crosses the blood-brain barrier and selectively binds to β-pleated sheets found in dense core Aβ plaques. It allows pre-screening of plaque-containing tissue sections prior to immunostaining and processing for electron microscopy.
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Bisht, K., El Hajj, H., Savage, J. C., Sánchez, M. G., Tremblay, M. Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques. J. Vis. Exp. (112), e54060, doi:10.3791/54060 (2016).
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