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Мышиные Hind Лимб длинных трубчатых костей Вскрытие и костного мозга Изоляция
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
DOI:

07:17 min

April 14, 2016

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Capítulos

  • 00:05Título
  • 00:41Hind Limb Long Bone Dissection
  • 03:01Long Bone Preparation For Bone Marrow Isolation
  • 04:25Bone Marrow Isolation
  • 05:29Results: Representative Downstream Analyses and Applications of Rapid DIssected Mouse Long Bones and Bone Marrow
  • 06:28Conclusion

Summary

Tadução automática

Here we present a protocol for the dissection of hind limb long bones (femurs and tibiae) from the laboratory mouse. We further describe a rapid technique for bone marrow isolation from these bones that utilizes centrifugation for removal of bone marrow from the bone marrow space.

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