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Imunologia e Infecção
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Selective Harvesting of Marginating-hepatic Leukocytes
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Selective Harvesting of Marginating-hepatic Leukocytes

Selective Harvesting of Marginating-hepatic Leukocytes

DOI:
10.3791/53918-v

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06:53 min

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July 21, 2016

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, , , ,
1Sagol School of Neuroscience & School of Psychological Sciences,Tel-Aviv University

Capítulos

  • 00:05Título
  • 00:55Liver Perusion and Marginating-hepatocyte (MH) Leukocyte Collection in Rats
  • 03:30Liver Perusion and MH Leukocyte Collection in Mice
  • 05:16Results: Cytotoxicity of MH-NK Cells and Whole Liver Leukocyte Populations
  • 06:04Conclusion

Summary

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Tadução automática

Marginating-hepatic leukocytes exhibit unique characteristics and distinct immunological functions compared to other leukocyte populations. Here we describe a method for selective harvesting of this specific hepatic cell population, through forced perfusion of the liver of rats or mice. Marginating-hepatic leukocytes seem critical in determining susceptibility to hepatic-related diseases and metastases.

Tags

Marginating-hepatic LeukocytesLiver SinusoidLiver PerfusionLeukocyte HarvestingLiver Immune CellsHepatic Leukocyte Isolation

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