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성인 피부 섬유 아 세포에서 인슐린 분비 세포를 얻을 수있는 안전하고 간단한 방법으로 성적인 변환
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts
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성인 피부 섬유 아 세포에서 인슐린 분비 세포를 얻을 수있는 안전하고 간단한 방법으로 성적인 변환

6,674 Views

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08:45 min

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March 18, 2016

DOI:

10.3791/53880-v

DOI:
10.3791/53880-v

•

08:45 min
March 18, 2016

•

3 Views
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1Laboratory of Biomedical Embryology, Unistem, Centre for Stem Cell Research,Università degli Studi di Milano

Tags

Epigenetic ConversionInsulin-secreting CellsAdult Skin FibroblastsRegenerative MedicineCell TherapyDiabetesPluripotencyRetroviral VectorsPorcine GelatinPBSAntibiotic AntimycoticSkin BiopsyFibroblast MediumCell MonolayerPassage RatioTrypsin-EDTACell SuspensionCounting Chamber

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Cite this Article

Brevini, T. A., Pennarossa, G., Maffei, S., Zenobi, A., Gandolfi, F. Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts. J. Vis. Exp. (109), e53880, doi:10.3791/53880 (2016).

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