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Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts

Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts

DOI:
10.3791/53880-v

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08:45 min

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March 18, 2016

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, , , ,
1Laboratory of Biomedical Embryology, Unistem, Centre for Stem Cell Research,Università degli Studi di Milano

Capítulos

  • 00:05Título
  • 00:50Skin Fibroblast Isolation
  • 01:53Fibroblast Culture and Plating for Epigenetic Conversion
  • 04:00Increase Cell Plasticity Using the De-methylating Agent 5-aza-CR
  • 05:31Pancreatic Differentiation Protocol
  • 06:47Results: Morphological and Functional Changes During Epigenetic Conversion
  • 08:02Conclusion

Summary

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Here, a new method that allows the conversion of adult skin fibroblasts into insulin-secreting cells is presented. This technique is based on epigenetic conversion, does not involve the use of retroviral vectors nor the acquisition of a stable pluripotent state. It is therefore highly promising for translational medicine applications.

Tags

Keywords: Epigenetic ConversionInsulin-secreting CellsAdult Skin FibroblastsRegenerative MedicineCell TherapyDiabetesPluripotencyRetroviral VectorsPorcine GelatinPBSAntibiotic AntimycoticSkin BiopsyFibroblast MediumCell MonolayerPassage RatioTrypsin-EDTACell SuspensionCounting Chamber

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