Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos

Giselbert Hauptmann; Iris S&#246;ll; Robert Krautz; Ulrich Theopold

doi:10.3791/53830

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Multi-Target-Chromogenic Whole-mount In-situ- Hybridisierung zum Vergleich Genexpression Domains in Drosophila Embryos

JoVE Journal
Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos

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Multi-Target-Chromogenic Whole-mount In-situ- Hybridisierung zum Vergleich Genexpression Domains in Drosophila Embryos

11,220 Views

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10:17 min

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January 31, 2016

DOI:

10.3791/53830-v

DOI:

10.3791/53830-v

•

10:17 min

January 31, 2016

•

7 Views

Giselbert Hauptmann¹, Iris Söll¹, Robert Krautz¹, Ulrich Theopold¹

¹Department of Molecular Biosciences, The Wenner-Gren Institute (MBW),Stockholm University

Summary

Wir beschreiben eine Multi-Target-chromogenen ganze-mount in situ Hybridisierung (MC-WISH) Verfahren in intakten Drosophila-Embryos eine gleichzeitige und spezifischen Nachweis von drei verschiedenen mRNA-Verteilungsmuster durch Kontrastfarbe Niederschläge.

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Multi-Target-Chromogenic Whole-mount<em> In-situ-</em> Hybridisierung zum Vergleich Genexpression Domains in<em> Drosophila</em> Embryos

Summary

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