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从培养儿童原发性鼻腔上皮细胞和重新编程为诱导多能干细胞
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
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从培养儿童原发性鼻腔上皮细胞和重新编程为诱导多能干细胞

10,950 Views

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12:08 min

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March 10, 2016

DOI:

10.3791/53814-v

DOI:
10.3791/53814-v

•

12:08 min
March 10, 2016

•

3 Views
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1Pyrosequencing Core,Cincinnati Children’s Hospital, 2Division of Developmental Biology,Cincinnati Children’s Hospital, 3Division of Pulmonary Medicine,Seattle Children’s Hospital, 4Division of Asthma Research,Cincinnati Children’s Hospital

Summary

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This publication demonstrates methods for successful sampling and culture of nasal epithelial mucosa from children, and reprogramming these cells to induced Pluripotent Stem Cells (iPSCs).

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Cite this Article

Ulm, A., Mayhew, C. N., Debley, J., Khurana Hershey, G. K., Ji, H. Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells. J. Vis. Exp. (109), e53814, doi:10.3791/53814 (2016).

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