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发育生物学
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Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
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发育生物学
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JoVE 杂志 发育生物学
Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells

DOI:
10.3791/53814-v

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12:08 min

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March 10, 2016

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, , , ,
1Pyrosequencing Core,Cincinnati Children’s Hospital, 2Division of Developmental Biology,Cincinnati Children’s Hospital, 3Division of Pulmonary Medicine,Seattle Children’s Hospital, 4Division of Asthma Research,Cincinnati Children’s Hospital

Chapters

  • 00:05Title
  • 01:13Sampling the Nasal Mucosa
  • 02:50Counting and Spinning Cells
  • 04:20Seeding Cells
  • 05:06Reprogramming to iPSCs
  • 09:11Results: Generation of iPSCs from Nasal Epithelial Cells
  • 10:47Conclusion

Summary

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This publication demonstrates methods for successful sampling and culture of nasal epithelial mucosa from children, and reprogramming these cells to induced Pluripotent Stem Cells (iPSCs).

Tags

Nasal Epithelial CellsInduced Pluripotent Stem CellsIPSCsAerated DiseaseAsthmaEpigenetic VariationAir PollutionNasal SamplingReprogrammingBEGMCytology BrushLive/dead Cell Stain

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