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Epicardico escrescenza Cultura Assay e<em> Ex Vivo</em> La valutazione della migrazione delle cellule di derivazione epicardico
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration
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Epicardico escrescenza Cultura Assay e<em> Ex Vivo</em> La valutazione della migrazione delle cellule di derivazione epicardico

8,038 Views

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07:44 min

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March 18, 2016

DOI:

10.3791/53750-v

DOI:
10.3791/53750-v

•

07:44 min
March 18, 2016

•

2 Views
, ,
1Aab Cardiovascular Research Institute,University of Rochester School of Medicine and Dentistry, 2Department of Medicine,University of Rochester School of Medicine and Dentistry, 3Department of Pharmacology and Physiology,University of Rochester School of Medicine and Dentistry

Summary

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Here, we describe methods for isolating primary mouse epicardial cells by an outgrowth culture assay and assessing the functional migration of epicardial-derived cells (EPDC) using an ex vivo heart culture system. These protocols are suitable for identifying genetic and chemical modulators of epicardial epithelial-to-mesenchymal transition (EMT) and motility.

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Trembley, M. A., Velasquez, L. S., Small, E. M. Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration. J. Vis. Exp. (109), e53750, doi:10.3791/53750 (2016).

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