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Imunologia e Infecção
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Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network

Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network

DOI:
10.3791/53736-v

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05:07 min

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February 16, 2016

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, , ,
1Oral Immunity and Inflammation Unit, NIDCR,NIH, 2Manchester Immunology Group, Faculty of Life Sciences,University of Manchester, 3Manchester Collaborative Centre for Inflammation Research,University of Manchester

Capítulos

  • 00:05Título
  • 00:45Gingival Block Isolation, Dissection, and Immune Cell Collection
  • 03:27Results: Representative Gingival Cell Cytokine Production
  • 04:25Conclusion

Summary

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We have established a technique for the isolation, phenotypic characterization and functional analysis of immune cells from murine gingiva.

Tags

IsolationCharacterizationFunctional ExaminationGingival Immune CellsOral ImmunityPeriodontitisImmune ResponsesCellular FactorsPathogenesisTechniqueOral Immune Cell NetworkViable CellsIn-vitro InvestigationsProtocolPinsThoracic And Abdominal CavitiesHeartInternal OrgansVena CavaPBS FlushMouse Head ImmobilizationDissecting PadStomach Facing UpScissorsCommissure Of The LipMandible And Neck RegionLower IncisorsSeparated Mandible Immobilization25-gauge NeedlesOral CavityPalate Identification

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