Rivista
/
Immunologia e infezioni
/
Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network

Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network

DOI:
10.3791/53736-v

•

05:07 min

•

February 16, 2016

•

, , ,
1Oral Immunity and Inflammation Unit, NIDCR,NIH, 2Manchester Immunology Group, Faculty of Life Sciences,University of Manchester, 3Manchester Collaborative Centre for Inflammation Research,University of Manchester

Capitoli

  • 00:05Titolo
  • 00:45Gingival Block Isolation, Dissection, and Immune Cell Collection
  • 03:27Results: Representative Gingival Cell Cytokine Production
  • 04:25Conclusion

Summary

Traduzione automatica

Traduzione automatica

We have established a technique for the isolation, phenotypic characterization and functional analysis of immune cells from murine gingiva.

Tags

IsolationCharacterizationFunctional ExaminationGingival Immune CellsOral ImmunityPeriodontitisImmune ResponsesCellular FactorsPathogenesisTechniqueOral Immune Cell NetworkViable CellsIn-vitro InvestigationsProtocolPinsThoracic And Abdominal CavitiesHeartInternal OrgansVena CavaPBS FlushMouse Head ImmobilizationDissecting PadStomach Facing UpScissorsCommissure Of The LipMandible And Neck RegionLower IncisorsSeparated Mandible Immobilization25-gauge NeedlesOral CavityPalate Identification

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/53736?article_type=v

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry
check_url/it/53736?article_type=v

Isolation of Functional Cardiac Immune Cells
check_url/it/53736?article_type=v

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
check_url/it/53736?article_type=v

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
check_url/it/53736?article_type=v

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
check_url/it/53736?article_type=v

Isolation, Processing and Analysis of Murine Gingival Cells
check_url/it/53736?article_type=v

Isolation of Adipose Tissue Immune Cells
check_url/it/53736?article_type=v

An In Vitro Model for Measuring Immune Responses to Malaria in the Context of HIV Co-infection
check_url/it/53736?article_type=v

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
check_url/it/53736?article_type=v

Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies

Read Article