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HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice
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Neurociência
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JoVE Journal Neurociência
HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice

HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice

DOI:
10.3791/53717-v

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07:30 min

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July 09, 2016

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, ,
1Department of Psychiatry,Icahn School of Medicine at Mount Sinai, 2Department of Neurology,Icahn School of Medicine at Mount Sinai, 3Friedman Brain Institute,Icahn School of Medicine at Mount Sinai, 4Department of Physiology and Biophysics,Virginia Commonwealth University Medical School

Capítulos

  • 00:05Título
  • 00:56Stereotaxic Surgery to Introduce HSV Chimeric Constructs
  • 03:32Head Twitch Response Experiment
  • 05:04Data Review
  • 05:33Resultados
  • 06:35Conclusion

Summary

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This article describes how to inject viral vectors into the mouse frontal cortex to test behavioral assays that require GPCR heteromeric formation.

Tags

HSV-Mediated Transgene ExpressionChimeric ConstructsBehavioral FunctionGPCR HeteromersMiceVirus-mediated Gene TransferBehavioral PhenotypesNeuro PharmacologyPsycho PharmacologyG-protein Coupled ReceptorsMolecular Level InteractionPsychiatric DisordersSchizophreniaDepressionSeratonin 2a ReceptorMetabotropic Glutamate Receptor 2Mechanism Of ActionDrugs For Schizophrenia TreatmentAnesthesiaSurgical Plane Of AnesthesiaOphthalmic GelStereotactic FrameScalp IncisionPureit Clamps

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