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Biologia

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

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Biologia

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JoVE Journal Biologia

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

DOI:

10.3791/53613-v

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05:44 min

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April 25, 2016

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Anne-Sophie Perrin-Terrin², Florine Jeton³, Aurelien Pichon^3,4, Alain Frugière, Jean-Paul Richalet³, Laurence Bodineau, Nicolas Voituron³

¹Sorbonne Paris Cité, Laboratory “Hypoxia & Lung” EA2363,University Paris 13, ²UPMC Univ Paris 06, INSERM, UMR_S1158 Neurophysiologie Respiratoire Expérimentale et Clinique,Sorbonne Universités, ³Laboratory of Excellence GR-Ex, ⁴Laboratory MOVE (EA 6314),University of Poitiers

Capítulos

00:05Título
00:44Day 1 Endogenous Peroxidase Activity Destruction, Non-specific Binding Site Blockade and Incubation with the Primary Antibody
01:44Day 3 Incubation with Secondary Antibody and Development of a Color Reaction
03:21Sample Mounting
04:16Results: c-FOS-positive Neurons on Sections of the Medulla Oblongata in Control Mice and Mice Submitted to Hypoxia In Vivo
05:17Conclusion

Summary

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Here, we present a protocol based on c-FOS protein immunohistological detection, a classical technique used for the identification of neuronal populations involved in specific physiological responses in vivo and ex vivo.

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

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