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Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
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Neurociência
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JoVE Journal Neurociência
Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

11,256 Views

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16:45 min

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March 13, 2016

DOI:

10.3791/53528-v

DOI:
10.3791/53528-v

•

16:45 min
March 13, 2016

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1Department of Molecular and Cellular Neuroscience,Nencki Institute of Experimental Biology, 2Department of Biochemistry,Nencki Institute of Experimental Biology, 3Neurobiology Centre,Nencki Institute of Experimental Biology

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Two-photon ImagingIn VivoRetrosplenial CortexCraniotomyAdeno-associated VirusStructural PlasticityGFPMCherryStereotactic FrameIsofluraneAnesthesiaInflammationPainInfection

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Łukasiewicz, K., Robacha, M., Bożycki, Ł., Radwanska, K., Czajkowski, R. Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex. J. Vis. Exp. (109), e53528, doi:10.3791/53528 (2016).

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