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Step-specific Sorting of Mouse Spermatids by Flow Cytometry
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Step-specific Sorting of Mouse Spermatids by Flow Cytometry
DOI:

06:31 min

December 31, 2015

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Capítulos

  • 00:05Título
  • 00:50Tube and Cell Preparation
  • 03:01Cell Sorting
  • 04:50Results: Validation of the Purification Scheme by Epifluorescence Microscopy
  • 05:46Conclusion

Summary

Tadução automática

We describe a sorting strategy for mouse spermatids using flow cytometry. Spermatids are sorted into four highly pure populations, including round (spermiogenesis steps 1-9), early elongating (spermiogenesis steps 10-12), late elongating (spermiogenesis steps 13-14) and elongated spermatids (spermiogenesis steps 15-16). DNA staining, size and granulosity are used as selection parameters.

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