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Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
JoVE Journal
Biologia
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JoVE Journal Biologia
Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

DOI:
10.3791/53268-v

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08:14 min

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November 13, 2015

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, , , , , , , ,
1Department of Cardiovascular Surgery, German Heart Center Munich,Technische Universität München, 2Institute for Medical Microbiology, Immunology, and Hygiene,Technische Universität München, 3Clinical Cooperation Groups: “Antigen-specific Immunotherapy” and “Immune Monitoring”, Helmholtz Center Munich (Neuhererg),Technische Universität München, 4DZHK (German Center for Cardiovascular Research) – Partner site Munich Heart Alliance

Capítulos

  • 00:05Título
  • 01:41Cell Culture Treatment with Nanoparticles
  • 03:42Identification of Cy3-positive Cells via Flow Cytometry
  • 05:53Results: Detection of Pluripotency Marker Gene Expression in Live iPSCs and Embryonic Stem Cells
  • 07:36Conclusion

Summary

Tadução automática

Tadução automática

This manuscript describes a novel technique which allows the detection of intracellular gene expression after endocytosis of fluorescence-labeled nanoparticles directly in live cells. The method does not require manipulation of the cells and is not restricted with respect to the target gene or species.

Tags

Keywords: Intracellular Gene ExpressionLive CellsFluorescence-labeled NanoparticlesInduced Pluripotent Stem CellsPluripotency GenesTranscription FactorsCell NucleusImmunohistochemical StainingGene-specific NanoparticlesTarget MRNAReporter StrandEndocytosisFlow Cytometry

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