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使用分离线粒体从小鼠骨骼肌进行高通量微孔板呼吸测量最小数量
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
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使用分离线粒体从小鼠骨骼肌进行高通量微孔板呼吸测量最小数量

13,231 Views

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10:39 min

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October 30, 2015

DOI:

10.3791/53216-v

DOI:
10.3791/53216-v

•

10:39 min
October 30, 2015

•

2 Views
, , , , ,
1The Department of Human Nutrition, Foods, and Exercise,Virginia Tech, 2The Metabolic Phenotyping Core,Virginia Tech, 3Seahorse Bioscience

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Key Words:Mitochondrial RespirationSkeletal MuscleMouseMicroplateHigh-throughputIsolated MitochondriaRespiratory Measurements

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Boutagy, N. E., Rogers, G. W., Pyne, E. S., Ali, M. M., Hulver, M. W., Frisard, M. I. Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements. J. Vis. Exp. (104), e53216, doi:10.3791/53216 (2015).

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