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Generation of Prostate Cancer Patient Derived Xenograft Models from Circulating Tumor Cells
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JoVE Journal Medicina
Generation of Prostate Cancer Patient Derived Xenograft Models from Circulating Tumor Cells

Generation of Prostate Cancer Patient Derived Xenograft Models from Circulating Tumor Cells

DOI:
10.3791/53182-v

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08:03 min

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October 20, 2015

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, , , , , , , ,
1Department of Pathology, Tisch Cancer Institute,Icahn School of Medicine at Mount Sinai, 2Department of Hematology/Oncology, Tisch Cancer Institute,Icahn School of Medicine at Mount Sinai, 3Molecular Biology Program,Memorial Sloan-Kettering Cancer Center

Capítulos

  • 00:05Título
  • 01:30Isolation of Mononuclear Cells
  • 03:42Staining Mononuclear Cells with CD45-FITC Antibody
  • 05:33Injection of Circulating Tumor Cells into Mice
  • 06:30Results: Generation of Prostate Cancer PDX Models
  • 07:36Conclusion

Summary

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Tadução automática

This manuscript details a method used to generate prostate cancer patient derived xenografts (PDXs) from circulating tumor cells (CTCs). The generation of PDX models from CTCs provides an alternative experimental model to study prostate cancer; the most commonly diagnosed tumor and a frequent cause of death from cancer in men.

Tags

Keywords: Prostate CancerPatient Derived XenograftPDXCirculating Tumor CellsCTCsMetastasisPesquisa do CâncerPreclinical Drug EvaluationBiomarker IdentificationPersonalized MedicineFlow CytometryImmunocompromised MiceMolecular Characterization

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