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Cryosectioning Method for Microdissection of Murine Colonic Mucosa
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Biologia
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JoVE Journal Biologia
Cryosectioning Method for Microdissection of Murine Colonic Mucosa

Cryosectioning Method for Microdissection of Murine Colonic Mucosa

16,815 Views

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06:16 min

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July 12, 2015

DOI:

10.3791/53112-v

DOI:
10.3791/53112-v

•

06:16 min
July 12, 2015

•

11 Views
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1Epithelial Pathobiology and Mucosal Inflammation Research Unit, Department of Pathology and Laboratory Medicine,Emory University, Atlanta, Georgia

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Here we describe a simple method for the isolation of spatially distinct murine colonic epithelial surface cells from the underlying crypt-base cells.

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CryosectioningMicrodissectionMurineColonic MucosaEpithelial CellsCrypts Of LieberkuhnProgenitor CellsCellular DifferentiationProliferative Crypt ZoneSurface Epithelial CellsRNA IsolationProtein Isolation

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Farkas, A. E., Gerner-Smidt, C., Lili, L., Nusrat, A., Capaldo, C. T. Cryosectioning Method for Microdissection of Murine Colonic Mucosa. J. Vis. Exp. (101), e53112, doi:10.3791/53112 (2015).

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