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Immunology and Infection

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JoVE Journal Immunology and Infection
Co-immunoprecipitation of the Mouse Mx1 Protein with the Influenza A Virus Nucleoprotein
 

Co-immunoprecipitation of the Mouse Mx1 Protein with the Influenza A Virus Nucleoprotein

Article DOI: 10.3791/52871-v • 09:39 min • April 21st, 2015
April 21st, 2015 •
1,2, 1,2, 1,2
1Inflammation Research Center, VIB, 2Department of Biomedical Molecular Biology, Ghent University

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This co-immunoprecipitation protocol allows to study the interaction between the influenza A virus nucleoprotein and the antiviral Mx1 protein in human cells. The protocol emphasizes the importance of N-ethylmaleimide for successful co-immunoprecipitation of Mx1 and influenza A virus nucleoprotein.

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Co-immunoprecipitation Protein Interaction Mx1 Protein Influenza A Virus Nucleoprotein Interaction Study Macromolecules Complex Sample Protocol Transfected Mammalian Cells Influenza A Virus Infected Cells Cell Lysis Specific Antibody Protein G Beads Western Blotting N-ethylmaleimide (NEM) Thiol Groups Stabilizes Interaction Conformation Third Component Limitation Contaminating Proteins Nonspecific Binding
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