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Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles
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Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles
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July 20, 2015
DOI:
1Department of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences,Radboud University Medical Center, 2Department of Biological Structure,University of Washington School of Medicine, 3Department of Biochemistry, Radboud Institute for Molecular Life Sciences,Radboud University Medical Center
Summary
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This protocol describes the isolation of satellite cells from branchiomeric head muscles of a 9 week-old rat. The muscles originate from different branchial arches. Subsequently, the satellite cells are cultured on a spot coating of millimeter size to study their differentiation. This approach avoids the expansion and passaging of satellite cells.
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Carvajal Monroy, P. L., Yablonka-Reuveni, Z., Grefte, S., Kuijpers-Jagtman, A. M., Wagener, F. A., Von den Hoff, J. W. Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles. J. Vis. Exp. (101), e52802, doi:10.3791/52802 (2015).
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