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隔离和卫星细胞的表征鼠头Branchiomeric肌肉
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July 20, 2015
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1Department of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences,Radboud University Medical Center, 2Department of Biological Structure,University of Washington School of Medicine, 3Department of Biochemistry, Radboud Institute for Molecular Life Sciences,Radboud University Medical Center
Summary
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这个协议描述了从一个9周龄大鼠branchiomeric头肌卫星细胞的分离。肌肉从不同的鳃弓起源。随后，卫星细胞在局部上光毫米大小来研究其分化培养。这种方法避免了卫星细胞的扩增和传代。
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Cite this Article
Carvajal Monroy, P. L., Yablonka-Reuveni, Z., Grefte, S., Kuijpers-Jagtman, A. M., Wagener, F. A., Von den Hoff, J. W. Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles. J. Vis. Exp. (101), e52802, doi:10.3791/52802 (2015).
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