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Electroretinogram Analysis of the Visual Response in Zebrafish Larvae
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Neurociência
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JoVE Journal Neurociência
Electroretinogram Analysis of the Visual Response in Zebrafish Larvae

Electroretinogram Analysis of the Visual Response in Zebrafish Larvae

DOI:
10.3791/52662-v

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09:44 min

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March 16, 2015

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, ,
1Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill, 2Department of Ophthalmology,Duke University

Capítulos

  • 00:05Título
  • 01:25Micropipette and Buffer Preparation
  • 02:16Electroretinogram Platform
  • 03:02Sponge and Electrode Preparation
  • 05:39Electroretinogram Analysis
  • 07:16Results: ERG Recording in 5 dpf Larval Zebrafish
  • 09:07Conclusion

Summary

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We present a method for the electroretinographic (ERG) analysis of zebrafish larvae utilizing micromanipulation and electroretinography techniques. This is a simple and straightforward method for assaying visual function of zebrafish larvae in vivo.

Tags

ElectroretinogramERGZebrafish LarvaeRetinal FunctionCone Visual FunctionIn VivoNoninvasive ElectrophysiologyGene SuppressionMorpholino OligonucleotidesPharmacological ManipulationStereomicroscopyElectrophysiology EquipmentLow Light CameraERG Stimulator/recorderZinc Finger Nucleases (ZFNs)Transcription Activator-Like Effector Nucleases (TALENs)Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9Pharmacological Agents

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