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엔테로 바이러스 및 문화와 RT-qPCR에 의해 물에서 노로 바이러스의 발생의 EPA 방법 1615 측정. 파트 III. RT-qPCR에 의한 바이러스 검출
JoVE Journal
Ambiente
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JoVE Journal Ambiente
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
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엔테로 바이러스 및 문화와 RT-qPCR에 의해 물에서 노로 바이러스의 발생의 EPA 방법 1615 측정. 파트 III. RT-qPCR에 의한 바이러스 검출

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12:32 min

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January 16, 2016

DOI:

10.3791/52646-v

DOI:
10.3791/52646-v

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12:32 min
January 16, 2016

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6 Views
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1National Exposure Research Laboratory,U.S. Environmental Protection Agency, 2Technical Services Center, Office of Ground Water and Drinking Water,U.S. Environmental Protection Agency

Tags

EPA Method 1615EnterovirusNorovirusRT-qPCRVirus DetectionWater SamplesCentrifugal UltrafiltrationNucleic Acid ExtractionReverse TranscriptionReal-time Thermal CyclerStandard CurveEnvironmental MicrobiologyRisk AssessmentDrinking WaterRecreational Water

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Fout, G. S., Cashdollar, J. L., Griffin, S. M., Brinkman, N. E., Varughese, E. A., Parshionikar, S. U. EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR. J. Vis. Exp. (107), e52646, doi:10.3791/52646 (2016).

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