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Enterovirus और संस्कृति और RT-qPCR द्वारा पानी में Norovirus घटना की ईपीए विधि 1615 मापन। भाग III। RT-qPCR द्वारा वायरस का पता लगाने
JoVE Journal
Ambiente
This content is Free Access.
JoVE Journal Ambiente
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
DOI:

12:32 min

January 16, 2016

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Capítulos

  • 00:05Título
  • 02:03Tertiary Concentration and Nucleic Acid Isolation
  • 03:21Reverse Transcription
  • 05:57Real Time Quantitative PCR
  • 09:23Results: RT-qPCR Determination of Enterovirus and Norovirus in Water
  • 11:38Conclusion

Summary

Tadução automática

Here we present a procedure to quantify enterovirus and norovirus in environmental and drinking waters using reverse transcription-quantitative PCR. Mean virus recovery from groundwater with this standardized procedure from EPA Method 1615 was 20% for poliovirus and 30% for murine norovirus.

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