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EPA方法1615测量肠道病毒和诺瓦克病毒发生水由文化和RT-qPCR的了。第三部分。病毒检测用RT-qPCR的
JoVE Journal
Ambiente
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JoVE Journal Ambiente
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
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EPA方法1615测量肠道病毒和诺瓦克病毒发生水由文化和RT-qPCR的了。第三部分。病毒检测用RT-qPCR的

DOI:
10.3791/52646-v

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12:32 min

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January 16, 2016

•

, , , , ,
1National Exposure Research Laboratory,U.S. Environmental Protection Agency, 2Technical Services Center, Office of Ground Water and Drinking Water,U.S. Environmental Protection Agency

Capítulos

  • 00:05Título
  • 02:03Tertiary Concentration and Nucleic Acid Isolation
  • 03:21Reverse Transcription
  • 05:57Real Time Quantitative PCR
  • 09:23Results: RT-qPCR Determination of Enterovirus and Norovirus in Water
  • 11:38Conclusion

Summary

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Here we present a procedure to quantify enterovirus and norovirus in environmental and drinking waters using reverse transcription-quantitative PCR. Mean virus recovery from groundwater with this standardized procedure from EPA Method 1615 was 20% for poliovirus and 30% for murine norovirus.

Tags

EPA Method 1615EnterovirusNorovirusRT-qPCRVirus DetectionWater SamplesCentrifugal UltrafiltrationNucleic Acid ExtractionReverse TranscriptionReal-time Thermal CyclerStandard CurveEnvironmental MicrobiologyRisk AssessmentDrinking WaterRecreational Water

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