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Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
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Biologia
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JoVE Journal Biologia
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

DOI:
10.3791/52484-v

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09:39 min

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March 17, 2015

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, ,
1Blood Systems Research Institute, 2Department of Medicine,University of California, San Francisco, 3Department of Laboratory Medicine,University of California, San Francisco

Capítulos

  • 00:05Título
  • 01:23Individual Extracellular Vesicle Detection
  • 05:33Bead-based Detection of EVs and Exosomes
  • 07:19Results: Representative Individual and Bead-based Detection Data
  • 08:49Conclusion

Summary

Tadução automática

Tadução automática

Many different methods exist for the measurement of extracellular vesicles (EVs) using flow cytometry (FCM). Several aspects should be considered when determining the most appropriate method to use. Two protocols for measuring EVs are presented, using either individual detection or a bead-based approach.

Tags

Keywords: Extracellular VesiclesFlow CytometryCell-cell CommunicationBiological ProcessesAnalysis TechniquesEV ProcessingBead-based ApproachIndividual Detection MethodSignal-to-noise RatioBackground FluorescenceClinical SamplesExosomes

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