This content is Free Access.
一个人类神经干细胞系的三维文化，微小RNA的分析和推测的靶基因分化
9,597 Views
•
10:48 min
•
April 12, 2015
DOI:
Summary
Automatically generated
With the intent of characterizing changes in miRNAs on differentiated human neural stem cells (hNSCs) we describe hNSC differentiation on a three dimensional system,the evaluation of changes in microRNA expression by miRNA PCR array, and computational analysis for miRNA target prediction and its validation by dual luciferase assay.
Read Article
Cite this Article
Stevanato, L., Hicks, C., Sinden, J. D. Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes. J. Vis. Exp. (98), e52410, doi:10.3791/52410 (2015).
Copy