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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes
 

Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes

Article DOI: 10.3791/52347-v • 09:21 min • December 27th, 2014
December 27th, 2014 •
1, 1, 1, 1, 1, 2, 3, 1, 1
1Department for Cardiology, Angiology and Pneumology, Otto von Guericke University Magdeburg, 2Herzzentrum Dresden, Universitätsklinikum an der Technischen Universität Dresden, Technische Universität Dresden, 3Department of Public Health and Primary Care, University of Cambridge

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Here we present a protocol that generates large amounts of murine monocytes from heterogeneous bone marrow for translational applications. In comparison to others, this new method helps reduce the number of sacrificed animals and lowers costs by avoiding expensive methods such as high gradient magnetic cell separation (MACS).

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Isolation Intravenous Injection Murine Bone Marrow Monocytes Leukocytes Macrophages Biomedical Science Isolation Method Balb/c Mice Flushing Phosphate Buffered Saline (PBS) Cell Suspension Macrophage-colony Stimulating Factor (M-CSF) Ultra-low Attachment Surfaces Adhesion-triggered Differentiation Characterization Phenotyping Fluorescence Activated Cell Sorting (FACS) CD11b CD115 F4/80 Markers Purity Adhesive Macrophages Removal Utilization Of Monocytes In Vivo Delivery Tail Vein Injection Mouse Model Studies Inflammation Immunology
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