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التمهيدي لالمناعية على نسيج Cryosectioned دماغ الفئران: مثال تلطيخ لالدبقية الصغيرة والخلايا العصبية

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

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التمهيدي لالمناعية على نسيج Cryosectioned دماغ الفئران: مثال تلطيخ لالدبقية الصغيرة والخلايا العصبية

31,051 Views

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07:30 min

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May 12, 2015

DOI:

10.3791/52293-v

DOI:

10.3791/52293-v

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07:30 min

May 12, 2015

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3 Views

Megan N. Evilsizor², Helen F. Ray-Jones³, Jonathan Lifshitz^2,4,5, Jenna Ziebell²

¹Department of Child Health,University of Arizona College of Medicine – Phoenix, ²BARROW Neurological Institute,Phoenix Children’s Hospital, ³Department of Biology and Biochemistry,University of Bath, ⁴Neuroscience Program,Arizona State University, ⁵Phoenix VA Healthcare System

Tags

Immunohistochemistry Cryosectioned Rat Brain Microglia Neurons Fluorescence Double-labeling Iba1 Pan-neuronal Visualization Protein Co-localization DAB Alkaline Phosphatase

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