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Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
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JoVE Journal Neurowissenschaften
Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

31,296 Views

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07:30 min

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May 12, 2015

DOI:

10.3791/52293-v

DOI:
10.3791/52293-v

•

07:30 min
May 12, 2015

•

29 Views
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1Department of Child Health,University of Arizona College of Medicine – Phoenix, 2BARROW Neurological Institute,Phoenix Children’s Hospital, 3Department of Biology and Biochemistry,University of Bath, 4Neuroscience Program,Arizona State University, 5Phoenix VA Healthcare System

Summary

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This introductory level protocol describes the reagents, equipment, and techniques required to complete immunohistochemical staining of rodent brains, using markers for microglia and neuronal elements as an example.

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ImmunohistochemistryCryosectionedRat BrainMicrogliaNeuronsFluorescenceDouble-labelingIba1Pan-neuronalVisualizationProtein Co-localizationDABAlkaline Phosphatase

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Cite this Article

Evilsizor, M. N., Ray-Jones, H. F., Lifshitz, J., Ziebell, J. Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons. J. Vis. Exp. (99), e52293, doi:10.3791/52293 (2015).

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