Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / TIRFM and pH-sensitive GFP-probes to Evaluate…
JoVE Journal
Neuroscience

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Neuroscience
TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells
 

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

Article DOI: 10.3791/52267-v • 13:47 min • January 29th, 2015
January 29th, 2015 •
1, 1, 1, 2, 1,3
1Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, 2San Raffaele Scientific Institute and Vita-Salute University, 3CEND Center of Excellence in Neurodegenerative Diseases, Università degli Studi di Milano

Capítulos

Resumo
Automatic Translation

This paper provides a method for investigating neurotransmitter vesicle dynamics in neuroblastoma cells, using a synaptobrevin2-pHluorin construct and Total Internal Reflection Fluorescence Microscopy. The strategy developed for image processing and data analysis is also reported.

Tags

TIRFM PH-sensitive GFP-probes Neurotransmitter Vesicle Dynamics SH-SY5Y Neuroblastoma Cells Cell Imaging Data Analysis Synaptic Vesicles Fusion And Retrieval Exo-endocytosis Real-time Monitoring Single-vesicle Level Genetically-encoded Probes Spatio-temporal Resolution Total Internal Reflection Fluorescence Microscopy Evanescent Field High-contrast Images Vesicle Tracking Fusion Events SH-SY5Y Cells Synapto-pHluorin Transfection
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter