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자료 쌍 해상도에서 DNA 메틸화의 평가에 대한 표현 바이 설 파이트 시퀀싱을 감소 향상
JoVE Journal
Biologia
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JoVE Journal Biologia
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
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자료 쌍 해상도에서 DNA 메틸화의 평가에 대한 표현 바이 설 파이트 시퀀싱을 감소 향상

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13:47 min

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February 24, 2015

DOI:

10.3791/52246-v

DOI:
10.3791/52246-v

•

13:47 min
February 24, 2015

•

18 Views
, , , , , , , ,
1Department of Medicine,Weill Cornell Medical College, 2Institute for Computational Biomedicine,Weill Cornell Medical College, 3Department of Physiology and Biophysics,Weill Cornell Medical College, 4Department of Pathology,University of Michigan

Summary

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Enhanced Reduced Representation Bisulfite Sequencing is a method for the preparation of sequencing libraries for DNA methylation analysis based on restriction enzyme digestion combined with cytosine bisulfite conversion. This protocol requires 50 ng of starting material and yields base pair resolution data at GC-rich genomic regions.

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Cite this Article

Garrett-Bakelman, F. E., Sheridan, C. K., Kacmarczyk, T. J., Ishii, J., Betel, D., Alonso, A., Mason, C. E., Figueroa, M. E., Melnick, A. M. Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution. J. Vis. Exp. (96), e52246, doi:10.3791/52246 (2015).

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