This content is Free Access.
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
Capítulos
- 00:05Título
- 02:22Adapter Ligation and DNA Size-selection via Gel Purification
- 07:32Bisulfite Conversion, Enrichment PCR, and DNA Purification
- 09:40Library Preparation for Sequencing
- 10:42Results: ERRBS Yields Base Pair Resolution CpG Methylation Data
- 12:15Conclusion
Enhanced Reduced Representation Bisulfite Sequencing is a method for the preparation of sequencing libraries for DNA methylation analysis based on restriction enzyme digestion combined with cytosine bisulfite conversion. This protocol requires 50 ng of starting material and yields base pair resolution data at GC-rich genomic regions.
