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浮袋注入による幼虫ゼブラフィッシュにおける粘膜カンジダ症のモデル化
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Modeling Mucosal Candidiasis in Larval Zebrafish by Swimbladder Injection
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浮袋注入による幼虫ゼブラフィッシュにおける粘膜カンジダ症のモデル化

10,002 Views

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06:28 min

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November 27, 2014

DOI:

10.3791/52182-v

DOI:
10.3791/52182-v

•

06:28 min
November 27, 2014

•

3 Views
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1Department of Molecular and Biomedical Sciences,University of Maine, 2Graduate School of Biomedical Sciences and Engineering,University of Maine

Summary

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In vivo spatio-temporal interactions of pathogen and immune defenses at the mucosal level are not easily imaged in existing vertebrate hosts. The method presented here describes a versatile platform to study mucosal candidiasis in live vertebrates using the swimbladder of the juvenile zebrafish as an infection site.

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Cite this Article

Gratacap, R. L., Bergeron, A. C., Wheeler, R. T. Modeling Mucosal Candidiasis in Larval Zebrafish by Swimbladder Injection. J. Vis. Exp. (93), e52182, doi:10.3791/52182 (2014).

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