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Use of Stopped-Flow Fluorescence and Labeled Nucleotides to Analyze the ATP Turnover Cycle of Kinesins
Capítulos
- 00:05Título
- 01:13Measurement of Association and Dissociation Rate Constants for mantATP
- 04:18Measurement of the Dissociation Rate Constant for mantADP
- 05:19Results: Analysis of the ATP Turnover Cycle of Kinesins
- 06:45Conclusion
Kinesins are characterized by nucleotide-dependent interaction with microtubules: a cycle of ATP turnover coupled to a cycle of microtubule interaction. Here, we describe protocols to analyze the kinetics of individual nucleotide transitions in the ATP turnover cycle of a kinesin using fluorescently labeled nucleotides and stopped-flow fluorescence.
